![]() Together this makes a 100 × 100-fold dilution, or 10,000-fold dilution. This mixture is then mixed well and 0.1 ml is removed from it into 9.9 ml of fresh diluent. In the above example, one could take 0.1 ml (100 μl) and add that volume to 9.9 ml of dilute. It is important to make sure that volumes are well mixed before drawing volumes to generate the next dilution, and that neither transfer devices nor vessels are reused prior to thorough rinsing or cleaning as well as, if necessary, sterilization. With serial dilutions, however, this same total dilution requires only five one-hundred-fold dilutions, such as 1 ml to 99 ml or 0.1 ml to 9.9 ml or even 0.01 ml (10 μl) to 0.99 ml, repeated five times and with the last four steps serving to further dilute the previously diluted volumes. If only a single step, however, then one would have to dilute 1 μl (one-thousandth of an ml) to 10 liters of diluent. This allows one to generate substantial levels of dilution without needing to employ very large volumes of diluent nor very small to-be- diluted volumes.įor example, it is quite simple to generate a ten-billion-fold dilution in only a handful of steps. ![]() With serial dilutions, dilutions take place one after another, with each subsequent dilution building upon the previous. Serial refers to events occurring in series, that is, one after another.
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